Description
NMRAL1 is a redox sensor protein that undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP+ levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. Recombinant human NMRAL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques